目的探讨金雀异黄素(Gen)对氧化损伤的人晶状体上皮细胞(HLEC)凋亡有无抑制作用。方法在终浓度3×10-4mol/L H2O2培养液中复制HLEC凋亡模型,并以雌二醇(E2)作为阳性对照,在透射电镜下观察HLEC超微结构的改变。结果超微结构研究显示,H2O2组绝大多数HLEC发生凋亡,并呈凋亡各期改变的全过程;E2、Gen组仅少数HLEC发生凋亡,并多为早期或中期的轻微改变。结论 Gen可减轻实验性氧化损伤的HLEC凋亡程度。 Objective To investigate whether Genistein can inhibit the apoptosis of human lens epithelial cells (HLECs) induced by oxidative stress. Methods HLEC apoptosis model was replicated in 3 × 10-4 mol / L H2O2 medium. Estradiol (E2) was used as a positive control and the ultrastructure of HLEC was observed under transmission electron microscope. Results Ultrastructural studies showed that most of the HLECs in the H2O2 group were apoptotic and showed the whole process of apoptosis. Only a few HLECs in E2 and Gen groups were apoptotic, and were mostly slightly changed in the early or middle stage. Conclusion Gen can reduce the apoptosis of HLEC induced by oxidative stress.