论文部分内容阅读
目的:探讨2型糖尿病(T2DM)并发干眼症患者结膜上皮细胞中核转录因子-κB(NF-κB)和基质金属蛋白酶-1(MMP-1)的表达及临床价值。方法:100例T2DM患者根据是否伴有干眼症分为T2DM并发干眼症组和单纯T2DM组,采用免疫印迹细胞化学检测结膜上皮细胞NF-κB和MMP-1的表达,并对NF-κB和MMP-1的表达做相关性分析。结果:T2DM并发干眼症组结膜上皮NF-κB阳性表达率为92.0%,明显高于单纯T2DM组(58.0%),相比较有差异(P<0.05);T2DM并发干眼症组结膜上皮MMP-1阳性表达率为90.0%,显著高于单纯T2DM组(46.0%),相比较有差异(P<0.05);T2DM并发干眼症组结膜上皮NF-κB和MMP-1的吸光值显著高于单纯T2DM组(P<0.05);T2DM并发干眼症患者结膜上皮细胞NF-κB表达与MMP-1表达呈显著正相关性(r=0.5491,P=0.017)。结论:T2DM并发干眼症患者结膜上皮细胞存在NF-κB和MMP-1的高表达,NF-κB和MMP-1的表达异常与临床上T2DM并发干眼症密切相关。
Objective: To investigate the expression of nuclear factor-κB (NF-κB) and matrix metalloproteinase-1 (MMP-1) in conjunctival epithelial cells of patients with type 2 diabetes mellitus (T2DM) complicated with dry eye. Methods: 100 T2DM patients were divided into T2DM group and T2DM group according to whether they had dry eye or not. The expressions of NF-κB and MMP-1 in conjunctival epithelial cells were detected by Western blot. And MMP-1 expression do correlation analysis. Results: The positive rate of NF-κB in conjunctival epithelium of T2DM patients with dry eye was 92.0%, which was significantly higher than that of T2DM patients (58.0%) (P <0.05) (P <0.05). The absorbance of NF-κB and MMP-1 in conjunctival epithelium of T2DM patients with dry eye was significantly higher than that of T2DM alone (46.0%) In T2DM group (P <0.05), there was a significant positive correlation between the expression of NF-κB and the expression of MMP-1 in conjunctival epithelial cells of patients with T2DM complicated with dry eye (r = 0.5491, P = 0.017). Conclusion: There is high expression of NF-κB and MMP-1 in conjunctival epithelial cells of T2DM patients with dry eye. The abnormal expression of NF-κB and MMP-1 are closely related to clinical T2DM complicated with dry eye.