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目的:研究志贺菌的耐药表型、携带的耐药基因及整合子的流行现状。方法:对2008年-2009年从闵行区腹泻病患者大便标本分离的63株志贺菌,用KB法测定其对9种抗生素的药物敏感情况;PCR筛查整合子种类;长片段扩增Ⅱ类整合子可变区并测序。结果:63株志贺菌均表现出明显的多重耐药和交叉耐药现象,福氏志贺菌对复方新诺明、氨苄青霉素、四环素和萘啶酸的耐药率达90%以上。宋内氏志贺菌对复方新诺明和四环素的耐药率达85%以上。92.1%(58/63)的志贺菌中检出II类整合子,未检出I类整合子。58株II类整合子保守区阳性的标本,可变区扩增亦全部阳性,大小约3 kb～4 kb之间。测序结果显示耐药菌株含有编码对磺胺类抗生素耐药的基因dfrA1、对链丝菌素耐药的基因sat1和对氨基糖甙类抗生素耐药的基因aadA1。结论:多重耐药和交叉耐药在志贺菌中广泛存在;耐药志贺菌携带II类整合子,II类整合子与耐药表型之间存在一定关联。
OBJECTIVE: To study the epidemic status of Shigella flexneri resistance phenotype, resistance gene and integron. Methods: Sixty-three Shigella isolated from stool samples of patients with diarrhea in Minhang District from 2008 to 2009 were tested for susceptibility to nine antibiotics by KB method. PCR screening of integron species was performed. The class integrates the variable regions and sequences. Results: 63 strains of Shigella showed obvious multi-drug resistance and cross-resistance. Shigella flexneri resistant to cotrimoxazole, ampicillin, tetracycline and nalidixic acid were more than 90%. Shigella sonnei against cotrimoxazole and tetracycline resistance rate of 85%. Type II integrons were detected in 92.1% (58/63) of Shigella strains and no class I integrons were detected. 58 strains of class II integron-conserved region-positive specimens, variable region amplification also all positive, the size of about 3 kb ~ 4 kb. Sequencing results showed that the resistant strains contained dfrA1 gene encoding streptozotocin resistance, sat1 gene resistant to streptozotocin and aadA1 gene resistant to aminoglycoside antibiotics. Conclusion: The multi-drug resistance and cross-resistance are widespread in Shigella. There is a correlation between the resistance of Shigella spp. Carrying class II integron and the class II integron and drug resistance phenotype.