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目的:观察双参口服液对长期应用醋酸泼尼松引起的大鼠皮肤不良反应的预防作用。方法:32只SPF级♂SD大鼠,随机分为正常对照组(NS)、醋酸泼尼松组(GC)、双参口服液高剂量组(GC+SSH)、双参口服液低剂量组(GC+SSL)。根据体重给药,88天后处死大鼠,取背部正中皮肤进行组织病理学检查,并用计算机图像分析系统定量分析生长表皮厚度和弹力纤维面积百分比;另取一定质量背部去毛皮肤测定单位皮肤中羟脯氨酸含量。结果:长期应用醋酸泼尼松大鼠生长表皮显著变薄,弹力纤维减少、断裂,羟脯氨酸含量减少等皮肤不良反应现象(P<0.01或P<0.05)。双参口服液用药组大鼠生长表皮增厚,弹力纤维面积百分比增大,羟脯氨酸含量增加,皮肤不良反应明显减轻(P<0.01或P<0.05)。结论:双参口服液在所用剂量下对长期应用醋酸泼尼松致大鼠皮肤不良反应有较好的预防作用,并呈一定量效关系。
Objective: To observe the preventive effect of Shuangshen oral liquid on long-term adverse reactions induced by prednisone acetate in rats. Methods: Thirty-two SPF male SD rats were randomly divided into four groups: normal control group (NS), prednisone acetate group (GC), high dose group of Shuangshen oral liquid (GC + SSH) (GC + SSL). According to the body weight, rats were killed after 88 days, the back of the middle of the skin for histopathological examination, and the use of computer image analysis system quantitative analysis of the growth of epidermal thickness and elastic fiber area percentage; the other to take a certain amount of back to the hair on the skin to determine the unit of the skin Proline content. Results: Long-term application of prednisone acetate rat skin epidermis was significantly thinning, decreased elastic fibers, rupture, hydroxyproline content and other skin adverse reactions (P <0.01 or P <0.05). The rats in Shuangshen oral liquid group had thickening of epidermis, increasing percentage of elastic fiber area, increasing of hydroxyproline content and adverse reaction of skin (P <0.01 or P <0.05). Conclusion: Shuangshen oral liquid has good preventive effect on long-term use of prednisone acetate-induced adverse reactions on the skin of rats at the dosage of dose-effect relationship.