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目的 探讨IFN α基因治疗人黑色素瘤的免疫学机理。方法 以人IFN α基因为治疗性目的基因 ,建立了人IFN α基因疗法治疗人黑色素瘤的实验模型 ,并检测机体的NK活性和巨噬细胞的杀伤活性。结果 高分泌IFN α成纤维细胞克隆株体内移植后 ,能显著提高机体的NK活性和巨噬细胞杀伤活性 ,在以IL 2为基础的过继疗法合用时 ,对NK活性和巨噬细胞杀伤活性的增强作用更加明显。结论 成纤维细胞介导的人IFN α基因疗法能显著增强机体的免疫功能 ,具有较强的抗黑色素瘤的作用 ,为临床应用细胞因子基因疗法治疗黑色素瘤提供了实验依据。
Objective To investigate the immunological mechanism of human melanoma treated with IFNα gene. Methods The human IFN α gene was used as therapeutic target gene to establish an experimental model of human IFN α gene therapy for human melanoma. The NK activity of the body and macrophage killing activity were detected. Results Highly secreted IFN α fibroblast clones could significantly increase NK activity and macrophage killing activity in vivo. When combined with IL 2 based adoptive therapy, the NK activity and macrophage killing activity The enhancement is even more pronounced. Conclusion The fibroblast-mediated human IFN alpha gene therapy can significantly enhance the body's immune function and has a strong anti-melanoma effect, providing experimental basis for the clinical application of cytokine gene therapy for the treatment of melanoma.