论文部分内容阅读
本文采用HPLC柱切换技术,建立了以YWG-C18(3cm)为预处理柱,乙酸(0.2mol/L)为预处理流动相,血浆直接进样。在线(on-line)固相净化血浆样品;以Shimpack CLC-ODS(15 cm)为分析柱,甲醇-0.2 mol/L乙酸铵缓冲液(14:86)为流动相,267 nm波长检测,采用外标法定量。测定H_2-受体拮抗剂法莫替丁人体血药浓度的方法。该法净化过程简便,为自动净化血浆样品提供了一选择方法。回收率好(81.10～84.75%);法莫替丁的检测限约为2.4 ng,血浆中最低检出浓度约为12 ng/ml。日内变异系数为4.1%,日间变异系数为4.9%;在浓度为15～210 ng/ml血浆范围内呈线性关系。
In this paper, HPLC column switching technology was used to establish a pretreatment column with YWG-C18 (3cm), acetic acid (0.2mol / L) as the pretreatment mobile phase, the direct injection of plasma. On-line solid-phase decontamination of plasma samples was performed on a Shimpack CLC-ODS (15 cm) column with methanol-0.2 mol / L ammonium acetate buffer (14:86) as the mobile phase at 267 nm. External standard method. Method for the determination of human plasma concentration of H 2 -receptor antagonist famotidine. The method of purification is simple and provides a choice method for automatically purifying plasma samples. The recovery rate was good (81.10-84.75%). The detection limit of famotidine was 2.4 ng and the lowest detectable concentration in plasma was 12 ng / ml. The intra-day coefficient of variation was 4.1% and the intra-day coefficient of variation was 4.9%. It was linear over a concentration range of 15-210 ng / ml.