论文部分内容阅读
目的观察4-苯基丁酸(4-PBA)对糖尿病大鼠心脏功能的影响并初步探讨其机制。方法♂Sprague-Dawley(SD)大鼠随机分成3组:对照组、糖尿病组和糖尿病+4-PBA组。单次腹腔注射链脲佐菌素(60 mg·kg-1)诱发大鼠糖尿病,在1型糖尿病模型制备成功后第5周开始每天给予大鼠4-PBA(1g·kg-1)灌胃,连续治疗20周。实验结束后,经右颈总动脉插管检测大鼠大动脉和左心室血流动力学变化,同时收集血清和心脏,比色法检测血糖,血清和心肌组织中超氧化物歧化酶(SOD)和一氧化氮合酶(NOS)活性,丙二醛(MDA)和一氧化氮(NO)含量。结果 4-PBA治疗对糖尿病大鼠血糖、体重和心脏重量无明显影响,但可改善糖尿病大鼠血流动力学,表现为大动脉收缩压和舒张压、心率、左心室收缩压和左心室内压最大上升和下降速率(±dp/dt)的轻度升高,左心室舒张压的轻度降低和舒张时程的缩短(P>0.05),左心室弛缓时间明显缩短(P<0.05)。4-PBA治疗尚可明显升高糖尿病大鼠血清和心肌组织SOD和NOS活力以及NO含量,并降低MDA含量(P<0.05)。结论 4-PBA治疗对糖尿病大鼠心脏有潜在保护作用,这一作用是通过改善糖尿病大鼠血流动力学和明显缓解其心脏舒张功能障碍而实现,并与其抑制动物体内氧化应激和促进心肌NO生成有关。
Objective To observe the effect of 4-phenylbutyric acid (4-PBA) on cardiac function in diabetic rats and to explore its mechanism. Methods ♂ Sprague-Dawley (SD) rats were randomly divided into three groups: control group, diabetes group and diabetes + 4-PBA group. Rats were induced by a single intraperitoneal injection of streptozotocin (60 mg · kg-1). Rats in type 1 diabetes mellitus were treated with 4-PBA (1 g · kg-1) , Continuous treatment for 20 weeks. At the end of the experiment, the hemodynamics of the aorta and left ventricle were detected by right common carotid artery cannula. Serum and heart were collected at the same time. The contents of superoxide dismutase (SOD) Nitric oxide synthase (NOS) activity, malondialdehyde (MDA) and nitric oxide (NO) content. Results 4-PBA treatment had no significant effect on blood glucose, body weight and heart weight in diabetic rats, but could improve hemodynamics in diabetic rats. Systolic and diastolic blood pressure, heart rate, left ventricular systolic pressure and left ventricular pressure (± dp / dt), slight decrease of left ventricular diastolic pressure and shortening of diastolic time course (P> 0.05), and the time of left ventricular relaxation was significantly shortened (P <0.05). 4-PBA treatment can still significantly increase the activity of SOD and NOS, the content of NO and the content of MDA in serum and myocardium of diabetic rats (P <0.05). Conclusions 4-PBA treatment may have a potential protective effect on the heart of diabetic rats by improving hemodynamics and relieving diastolic dysfunction in diabetic rats, and in combination with its inhibition of oxidative stress in animals and promotion of myocardial NO generation related.