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为探索烟粉虱的β-1,3-葡聚糖识别蛋白(BtβGRP)在烟粉虱先天免疫过程中的重要作用,对烟粉虱中鉴定的3个BtβGRPs基因编码的蛋白质进行结构分析,并与其它物种的23个同源蛋白进行系统进化分析,同时通过实时荧光定量PCR(quantitative real-time PCR,q RT-PCR)检测分析球孢白僵菌侵染烟粉虱不同虫态不同时间点后BtβGRPs的时间表达模式。结果显示:经鉴定3个BtβGRPs蛋白序列均含有βGRP家族特有的保守性结构域,即糖苷水解酶活性结构域;且3个BtβGRPs独自聚在进化树的一支上,可能参与烟粉虱特有的级联路径;3个BtβGRPs基因在不同虫态和不同诱导时间点与对照相比,除BtβGRP3在卵期未表达外,均有上调表达趋势,但诱导程度不同;成虫诱导24~36 h后达到峰值,比卵和若虫稍晚,可能是由于烟粉虱成虫体表有白色蜡粉等物质,延迟了球孢白僵菌对虫体的侵染。研究表明3个BtβGRPs蛋白可能参与了烟粉虱对真菌侵染的免疫反应,有可能成为烟粉虱生防的新靶标。
In order to explore the important role of β-1,3-glucan recognition protein (BtβGRP) of Bemisia tabaci (Gennadius) in innate immunity of Bemisia tabaci (Gennadius), structural analysis was performed on the three proteins identified by BtβGRPs, Phylogenetic analysis of 23 homologous proteins from other species was carried out. At the same time, the quantitative real-time PCR (q RT-PCR) Time after BtβGRPs expression pattern. The results showed that all the three BtβGRPs sequences contained the conserved domain of βGRP family, namely the glycoside hydrolase activity domain. Three BtβGRPs clustered independently in one of the phylogenetic trees, Compared with the control, all the three BtβGRPs showed an up-regulated expression trend but different degrees of induction except for BtβGRP3 at different stages and different induction time points. The adult BtβGRPs reached the level of 24-36 h Peak, slightly later than eggs and nymphs, may be due to the white wax powder body and other substances on the body surface of Bemisia tabaci, delay Beauveria bassiana infestation of parasites. Studies have shown that three BtβGRPs proteins may be involved in the immune response of the whitefly to fungal infection, which may become a new target of biocontrol of whitefly.