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最常用的精液制备技术是离心上游法(CS)。另一种较新的方法即在精子上游的培养中加粘稠度较高的透明质酸钠(SH),使活动好的精子无需离心自己即能移动穿过高粘稠度的培养液。作者比较了两法去除精液中微生物的能力。用不育夫妇中的20位丈夫精液,禁欲3天以上,手淫取精,1小时内送检。标本至少4ml以上才用于研究。标本液化后作精子计数,然后取1ml或用CS法,或用SH法制备后用于治疗性授精;再取1ml作细菌计数分析;剩下部分精液各1ml分别用CS、SH法制备,然后作精子计数;洗涤的精液标本放在37℃5%CO_2中孵育2小时和24小时作细菌培养。在前15份精液培养液中加入青霉素,余
The most commonly used semen preparation technique is centrifugation upstream (CS). Another newer approach is to add higher-viscosity sodium hyaluronate (SH) to sperm upstream so that motile spermatozoa can move through highly viscous broth without centrifugation. The authors compared the ability of two methods to remove microorganisms in the semen. 20 infertile couples with her husband’s semen, abstinence for more than 3 days, masturbation fine, within one hour submission. Specimens at least 4ml above for research. Sperm count after liquefaction, and then take 1ml or CS method, or SH method for the preparation of therapeutic insemination; then take 1ml for bacterial count analysis; the remaining part of the semen 1ml were prepared by CS, SH method, and then Sperm count; washed semen samples were incubated at 37 ℃ 5% CO 2 for 2 hours and 24 hours for bacterial culture. In the first 15 semen culture broth added penicillin, Yu