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AIM To observe the gene expression change ofeNOSmRNA and iNOSmRNA in the small and largeintestines with acute liver failure(ALF),and to reveal thebiological function of NO on the pathogenesis of ALF andmultiple organs dysfunction at the molecular level.METHODS Sixty male Wistar rats were selected,weighing from 250g to 350g,and divided into 5 groupsrandomly:SO,ALF(6h,12h),L-Arg,L-NAME,L-Arg andL-NAME,each group with 10 rats.The dose of L-Arg was300mg.kg~(-1),and L-NAME was 30mg.kg~(-1),the reagentsdiluted by normal saline were injected through tail vein 30minutes pre-and post-operation.The rats in the ALFgroup were respectively sacrificed postoperatively at 6h,12h,and the rats in the other groups were sacrificedpostoperatively at 6h.The tissues of small and largeIntestines were harvested in 4% psraforaldehydecontaining the reagent of DEPC and fixed at 6h,embeddedin paraffin,and 4μm section was cut.The expression ofeNOSmRNA and INOSmRNA in these tissues wasdetermined with in situ hybridization,and analyzed withthe imaging analysis system of CMM-3 and SPSSstatistical software.RESULTS The expression of eNOSmRNA in the largeIntestine and INOSmRNA in the small and large intestinesIncreased significantly at 6h after ALF,but the expressionof iNOSmRNA in the small and large intestines reducednotably at 12h after ALF(P<0.05);the expression ofeNOSmRNA in the large intestine and iNOSmRNA in thesmall and large intestines decreased significantly with thereagents of L-Arg at 6h ALF,but the expression ofeNOSmRNA and iNOSmRNA in the small and largeintestines decreased totally with the reagents of L-NAMEor association with L-Arg 6h ALF.CONCLUSION The expression of eNOSmRNA in the largeintestine increased notably at the early stage of ALF,NOinduced by the enzyme of eNOS from the transplantationof eNOSmRNA can protect the function of the largeintestine,the high expression of iNOSmRNA is involved inthe damaged function of the small and large intestines.NOprecursor can reduce the expression of iNOSmRNA in the small and large intestines and the damage to intestines;NOS inhibitor or association with NO precursor can totallylower the expression of eNOSmRNA and iNOSmRNA in thesmall and large intestines,it cannot notably influence theNOS inhibitor in the gene expression of eNOSmRNA andiNOSmRNA to supply the additional NO precursor.
AIM To observe the gene expression change of eNOS mRNA and iNOS mRNA in the small and largeintestines with acute liver failure (ALF), and to reveal thebiologicalfunction of NO on the pathogenesis of ALF and multiple organ dysfunction at the molecular level. METHODS Sixty male Wistar rats were selected , weighing from 250g to 350g and divided into 5 groups and randomly: SO, ALF (6h, 12h), L-Arg, L-NAME, L-Arg and L-NAME, each group with 10 rats. .kg ~ (-1), and L-NAME was 30mg.kg ~ (-1), the reagentsdiluted by normal saline were injected through the tail vein 30minutes pre-and post-operation.The rats in the ALFgroup were respectively sacrificed postoperatively at 6h, 12h, and the rats in the other groups were sacrificedpostoperatively at 6h. Tissues of small and large Intestines were harvested in 4% psraforaldehydecontaining the reagent of DEPC and fixed at 6h, embeddedin paraffin, and 4μm section was cut. The expression of eNOS mRNA and INOS mRNA in these tissues was determined with in situ hybrid ization, and analyzed with the imaging analysis system of CMM-3 and SPS Statistical software. RESULTS The expression of eNOS mRNA in the large In testine and INOS mRNA in the small and large intestines Initially significantly at 6h after ALF, but the expression of iNOS mRNA in the small and large intestines reduced notably at 12 h after ALF (P <0.05); the expression of eNOS mRNA in the large intestine and iNOS mRNA in the small andstatistically decreased significantly with agents of L-Arg at 6 h ALF, but the expression of eNOS mRNA and iNOS mRNA in the small and large intestines decreasing totally with the reagents of L-NAMEor association with L-Arg 6h ALF. CONCLUSION The expression of eNOS mRNA in the large intestine increased notably at the early stage of ALF, NOinduced by the enzyme of eNOS from the transplantation of eNOS mRNA can protect the function of the large intestine, the high expression of iNOS mRNA is involved inthe damaged function of the small and large intestines. NOprecursor can reduce the expression of iNOS mRNA in the small and large intestines and the damage to intestines; NOS inhibitor or association with NO precursor can totallylower the expression of eNOS mRNA and iNOS mRNA in thesmall and large intestines, it can not notably affect theNOS inhibitor in the gene expression of eNOS mRNA and iNOS mRNA to supply the additional NO precursor.