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我们应用ＭＫＮ45胃癌细胞株通过四唑蓝 (ＭＴＴ)比色分析法及流式细胞仪观察胃泌素在细胞增殖、凋亡中的作用 ,同时观察丙谷胺对胃泌素的拮抗作用 ,并与 5 氟尿嘧啶 (5 Ｆｕ)作比较 ,结果报道如下。一、材料与方法1.细胞培养 :ＭＫＮ45胃癌细胞株(上海市消化疾病研究所提供
Using MTT colorimetric assay and flow cytometry, we used MKN45 gastric cancer cell line to observe the effects of gastrin on cell proliferation and apoptosis, and to observe the effect of proglumide on gastrin antagonism. Compared with 5 Fu, the results are reported below. I. Materials and methods 1. Cell culture: MKN45 gastric cancer cell line (provided by Shanghai Institute of Digestive Diseases