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目的 :探讨细胞凋亡和细胞增殖在胃癌发生发展中的作用。方法 :用原位末端标记法和免疫组织化学染色 ,检测 30例胃癌和 2 0例胃粘膜上皮异型增生中的细胞凋亡和调控基因 (Bcl 2 ,Fas)及增殖细胞核抗原 (PCNA)的表达情况。结果 :随正常胃粘膜胃粘膜异型增生胃癌的梯度 ,细胞凋亡指数(AI)逐渐降低 ,差异有显著性 (P <0 0 5) ;Bcl- 2的阳性表达率逐渐升高 ,Fas的阳性表达率逐渐降低 ,差异均有显著性 (P <0 .0 1) ,且二者有相关性 (P <0 0 5)。PCNA阳性表达率 (即增殖指数 ,PI)逐渐升高 (P <0 .0 1)。结论 :胃粘膜异型增生中已存在细胞凋亡和细胞增殖的异常 ,使增殖 /凋亡比值加大 ,是胃粘膜异型增生向胃癌发展的重要机制之一
Objective : To explore the role of apoptosis and cell proliferation in the development of gastric cancer. METHODS: Apoptosis and expression of regulatory genes (Bcl 2 ,Fas) and proliferating cell nuclear antigen (PCNA) were detected in 30 cases of gastric cancer and 20 cases of gastric epithelial dysplasia by in situ end labeling and immunohistochemical staining. Happening. RESULTS: With the gradient of normal gastric mucosa, gastric dysplasia and gastric cancer, the apoptosis index (AI) gradually decreased (P < 0.05); the positive expression rate of Bcl-2 gradually increased. The positive expression rate gradually decreased, the difference was significant (P < 0.01), and the two were related (P <0 05). The positive rate of PCNA (ie, proliferation index, PI) gradually increased (P < 0.01). Conclusion: Abnormalities of cell apoptosis and cell proliferation have existed in dysplasia of gastric mucosa, and the ratio of proliferation/apoptosis has been increased. It is one of the important mechanisms of development of gastric dysplasia to gastric cancer.