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目的检测急性加重期慢性阻塞性肺疾病(AECOPD)患者外周血中性粒细胞凋亡率,观察N-乙酰半胱氨酸对其影响。方法40例AECOPD患者分为2组:观察组20例,服用N-乙酰半胱氨酸(1次200mg,1日3次);常规治疗组20例。另设正常对照组18例。流式细胞仪检测中性粒细胞凋亡率。结果观察组和常规治疗组AECOPD患者治疗前中性粒细胞凋亡率均明显低于正常对照组〔(22.71±11.92)%vs(54.32±13.01)%,P<0.01;(20.04±7.05)%vs(54.32±13.01)%,P<0.01〕,提示AECOPD患者中性粒细胞凋亡延迟。治疗后中性粒细胞凋亡率均有所增加,但观察组与常规治疗组比较无显著性差异(P>0.05)。结论AECO-PD患者中性粒细胞凋亡延迟,N-乙酰半胱氨酸短期治疗对其无明显影响。
Objective To detect the apoptosis rate of peripheral blood neutrophil in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and to observe the effect of N-acetylcysteine on it. Methods Forty patients with AECOPD were divided into two groups: 20 in the observation group, N-acetylcysteine (200 mg once, three times a day) in the observation group and 20 in the conventional treatment group. Another set of normal control group of 18 cases. Flow cytometry was used to detect neutrophil apoptosis rate. Results The apoptotic rates of AECOPD patients in observation group and conventional treatment group were significantly lower than those in normal control group [(22.71 ± 11.92)% vs (54.32 ± 13.01)%, P <0.01; (20.04 ± 7.05)%, vs (54.32 ± 13.01)%, P <0.01〕, suggesting that AECOPD patients with delayed neutrophil apoptosis. After treatment, neutrophil apoptosis rate increased, but the observation group and the conventional treatment group showed no significant difference (P> 0.05). Conclusion The apoptosis of neutrophils in AECO-PD patients is delayed, and N-acetylcysteine has no obvious effect on it.