以1,8-二乙炔基芘和8-氨基喹啉为原料,通过click反应合成了一种双取代芘荧光探针L,表征了其结构.探针L可以在THF/H_2O(V∶V=1∶1,HEPES,1×10~(-2) mol/L,p H=7.4)中快速识别Cu~(2+),其他离子没有干扰.Job’s实验和高分辨质谱证明L与Cu~(2+)的结合计量比为1∶1,密度泛函理论计算表明L与Cu~(2+)形成了多元配合物.此外,L-Cu~(2+)对PPi具有良好的选择性和灵敏度,且响应快速,其检测限可达到6.32μmol·L~(-1),有潜在的应用价值. A novel disubstituted pyrene fluorescent probe L was synthesized by click reaction using 1,8-diethynylpyrene and 8-aminoquinoline as starting materials, and its structure was characterized. Probe L can be synthesized in THF / H 2 O (V: V Cu 2+ and other ions were not interfered by JE’s experiments and high-resolution mass spectrometry, which proved that L and Cu ~ (2+) is 1: 1, and density functional theory calculations show that L and Cu 2+ form multiple complexes. In addition, L-Cu 2+ has good selectivity for PPi And the sensitivity, and fast response, the detection limit of up to 6.32μmol·L -1, has potential applications.