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目的 探讨 p33ING1基因蛋白在胃癌中表达的临床意义及其相互关系。方法 应用Envision免疫组化法检测71例胃癌、12例胃粘膜不典型增生患者p33ING1、p5 3基因蛋白的表达 ,并与正常胃粘膜及慢性胃炎粘膜 18例对照。结果 粘膜不典型增生组及对照组中 p33ING1均阳性表达 (10 0 % ) ,而胃癌中表达 6 2 .0 % (4 4/ 71) ,明显下降 ,与前两组之间差异显著 (P <0 .0 1) ;胃癌中 p33ING1表达与肿瘤的浸润、淋巴结的转移及分化有关 (P <0 .0 1) ;p5 3表达与肿瘤大小、浸润、淋巴结转移有关 (P <0 .0 1)。结论 p33ING1是抑癌基因 ,在胃癌中表达下降 ,对胃癌发生、发展可能起重要作用 ,p33ING1和 p5 3在胃癌中表达有相关性 (P <0 .0 5 )。p33ING1与 p5 3野生型基因互相协同、依赖 ,抑制细胞生长 ,促进凋亡。检测胃癌患者p33ING1的水平 ,对p5 3介导的基因治疗有重要意义。
Objective To investigate the clinical significance of p33ING1 gene protein expression in gastric cancer and its relationship. Methods Envision immunohistochemical method was used to detect the expression of p33ING1 and p5 3 protein in 71 cases of gastric cancer and 12 cases of atypical hyperplasia of gastric mucosa, and compared with 18 cases of normal gastric mucosa and chronic gastritis mucosa. Results The positive expression of p33ING1 in mucosa dysplasia group and control group was 100%, while it was 62.0% (44/71) in gastric cancer, which was significantly lower than that in the former two groups (P < (P <0.01). The expression of p33ING1 in gastric cancer was related to tumor invasion, lymph node metastasis and differentiation (P <0.01). The expression of p53 was correlated with tumor size, invasion and lymph node metastasis (P <0.01) . Conclusion p33ING1 is a tumor suppressor gene, which is down-regulated in gastric cancer and may play an important role in the occurrence and development of gastric cancer. There is a correlation between p33ING1 and p5 3 in gastric cancer (P <0.05). p33ING1 and p5 3 wild-type genes cooperate and depend on each other, inhibiting cell growth and promoting apoptosis. Detection of p33ING1 levels in gastric cancer patients, p5 3-mediated gene therapy is important.