糖蛋白是胆色素结石的重要组分。但直接测定胆石或胆汁中糖蛋白含量的方法尚未见报告。Womack、Freston等用Boas法测定胆石或胆汁中的氨基己糖来作为糖蛋白的间接定量指标,研究其在胆固醇类胆石形成中的作用。我们发现Boas法用于测定胆石和胆汁中的氨基己糖时,受到胆石或胆汁中金属离子的干扰,而使其准确性大大降低。本文报告我们对Boas法进行改良以提高其准确性,和用 Glycoprotein is an important component of bile pigment stones. However, direct determination of gallstone or bile glycoprotein content has not been reported. Womack, Freston and other Boas method determination of gallstone or bile with hexokinase as an indirect quantitative indicators of glycoprotein to study its role in cholesterol gallstone formation. We found that when the Boas method was used to determine hexose in gallstones and bile, the accuracy of the Boas method was greatly diminished by the interference of metal ions in gallstone or bile. This article reports the Boas method we have improved to improve its accuracy, and use