为探索各种常见念珠菌的致病作用,我们共做的六种常见念珠菌对动物的致病作用,现报导如下: 材料和方法 1.念珠菌悬液的制备:将六种念珠菌分别接种子沙保劳氏琼脂斜面37℃培养24小时后,刮取菌苔于生理盐水中,稀释成25万个菌细胞/ml,再将此悬液的一部分放80℃水浴加温30分钟,常规培养确无活菌生长时,作对照组注射用悬液。 In order to explore the pathogenic effects of various common Candida species, the common pathogenic effects of six common Candida species on animals are reported below: Materials and Methods 1. Preparation of Candida suspensions: Six Candida species were isolated The inoculant Shabao’s agar slants were cultured at 37°C for 24 hours, scraped the bacteria in saline, and diluted to 250,000 bacteria cells/ml. A portion of this suspension was placed in a 80°C water bath and warmed for 30 minutes. When the conventional culture did not grow viable bacteria, it was used as a suspension for injection in the control group.