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目的:确定微波(1250MHz)灭活犬骨的最佳功率及微波灭活后的修复机理和需要的时间.方法:采用本地健康犬,分为即时、长期两组.即时组用微波单极天线,不同功率及时间介入辐射后即时取材.长期组用微波单极天线同一功率时间作用,术后不同时间取材,所有标本进行光镜、碱性磷酸酶特染、硝酸银染色、墨汁动脉灌注、四环素双标记.结果:①大功率短时间与中小功率长时间灭活距离无显著差异.②术后2周坏死区骨陷窝全部空虚,髓腔血运破坏,软组织及骨膜与骨质分离.术后12周,骨膜恢复,增生活跃,软组织附贴骨壁,髓腔血运完全再通,爬行替代大部完成.术后24周,爬行替代全部完成,骨修复与骨塑形同时进行.术后36周,骨塑形尚未完成.结论:临床应采用中小功率长时间作用方式为佳,灭活骨的修复至少三个月以上.
Objective: To determine the optimal power for inactivation of dog bone in microwave (1250 MHz) and the repair mechanism and time required after microwave inactivation. Methods: The local healthy dogs were divided into two groups: instant and long-term. The instant microwave microwave monopole antenna is used for instant data collection after different power and time intervention. For the long-term group, the microwave monopole antenna was used for the same power time, and the samples were taken at different times after operation. All the specimens were subjected to light microscopy, alkaline phosphatase staining, silver nitrate staining, ink arterial infusion, and tetracycline double labeling. Results: 1 There was no significant difference in long-term inactivation distance between high power and medium and small power. 2The bone lacunae in the necrotic area were completely empty 2 weeks after operation, and the blood flow in the medullary cavity was destroyed. The soft tissue and the periosteum were separated from the bone. At 12 weeks after surgery, the periosteum was recovered and the hyperplasia was active. The soft tissue adhered to the bone wall and the medullary blood flow was completely recanalized. Most of the crawling replacements were completed. At 24 weeks after surgery, reptile replacement was completed and bone repair was performed simultaneously with bone shaping. At 36 weeks after surgery, bone shaping was not completed. Conclusion: It is better to use medium and small power for a long time and the repair of inactivated bone should be at least three months.