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构建内皮祖细胞捕获支架是目前心血管疾病治疗的一个研究热点.利用磁性纳米粒子捕获内皮祖细胞也越来越受到关注,但是单纯的磁性纳米粒子和磁场源地作用相对微弱,本研究引入铁支架作为另一磁响应源,共同作用来提高纳米粒子表面捕获率.首先利用CD34抗体构建了一种定向捕获CD34阳性细胞的功能型磁性纳米粒子,再利用外加磁场将其归巢到铁支架表面.FTIR和TEM结果表明CD34抗体已成功接枝到双羧基PEG包裹的Fe_3O_4纳米粒子上,体外细胞评价证实该纳米粒子不具有细胞毒性.体内动物实验结果表明该Fe_3O_4-PEG@CD34纳米粒子能够特异性识别捕获CD34阳性细胞,并且在外加磁场的作用下能够将其快速归巢到铁支架表面,这为实现支架表面快速内皮化提供了可能.
The construction of endothelial progenitor cell capture scaffolds is currently a hot research topic in the treatment of cardiovascular diseases.It is also paid more and more attention to capture endothelial progenitor cells by using magnetic nanoparticles. However, the effect of simple magnetic nanoparticles and magnetic field sources is relatively weak. As another magnetic source, the scaffolds work together to enhance the surface capture rate of the nanoparticles.Firstly, a functional magnetic nanoparticle targeting CD34 positive cells was constructed by using CD34 antibody and then hurled to the surface of the iron scaffold by external magnetic field The results of FTIR and TEM indicated that the CD34 antibody was successfully grafted on the Fe_3O_4 nanoparticles coated with dicarboxy-PEG, and the cytotoxicity of the nanoparticles was not observed in vitro.Experimental results in vivo showed that the Fe_3O_4-PEG @ CD34 nanoparticles could specifically CD34 positive cells were captured by sexual recognition, and quickly homing to the surface of the iron stent under the applied magnetic field, which provided the possibility of rapid endothelialization of the scaffold surface.