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目的研究模拟微重力(simulated microgravity,SMG)条件下奥金肽(osgentide,OST)对小鼠前成骨细胞MC3T3-E1增殖功能的影响。方法在正常细胞培养环境下,利用MTT法检测6种OST化合物对MC3T3-E1细胞增殖的影响,从中筛选出有效作用浓度的化合物,进一步利用MTT实验及流式细胞术分别检测SMG条件下1 nmol/L OST5对MC3T3-E1细胞的增殖效应及细胞周期的分布。结果在正常细胞培养环境下,1 nmol/L OST5对MC3T3-E1细胞的增殖具有显著促进作用(P<0.01)。MTT实验结果表明,与正常细胞培养环境相比,MC3T3-E1细胞在SMG条件下其增殖受到显著抑制。在SMG条件下,用1 nmol/L OST5处理MC3T3-E1细胞(OST-SMG)3d,流式细胞术检测结果表明,SMG促使更多的MC3T3-E1细胞进入G1期。1 nmol/L OST5处理MC3T3-E1细胞后S期比例比SMG条件下显著提高(P<0.05),表明OST5能够促进DNA合成。结论在SMG条件下,OST5能够促进成骨细胞的增殖,为研究OST5对模拟微重力相关的骨丢失防治提供了理论依据。
Objective To study the effect of osgentide (OST) on the proliferation of MC3T3-E1 pre-mouse osteoblasts under simulated microgravity (SMG) conditions. Methods The effects of 6 OST compounds on the proliferation of MC3T3-E1 cells were detected by MTT assay under normal cell culture conditions. Compounds with effective concentrations were screened out by MTT assay. MTT assay and flow cytometry were used to detect the expression of 1 nmol / L OST5 on MC3T3-E1 cell proliferation and cell cycle distribution. Results Under normal cell culture conditions, 1 nmol / L OST5 significantly promoted the proliferation of MC3T3-E1 cells (P <0.01). The results of MTT assay showed that MC3T3-E1 cells significantly inhibited the proliferation of MC3T3-E1 cells under SMG conditions compared with normal cell culture environment. MC3T3-E1 cells (OST-SMG) were treated with 1 nmol / L OST5 for 3 days under SMG conditions. The result of flow cytometry showed that SMG promoted more MC3T3-E1 cells to enter G1 phase. The proportion of S phase in MC3T3-E1 cells treated with 1 nmol / L OST5 was significantly higher than that in SMG (P <0.05), indicating that OST5 can promote DNA synthesis. Conclusion Under the condition of SMG, OST5 can promote the proliferation of osteoblasts, providing a theoretical basis for the study of prevention and treatment of bone loss associated with simulated microgravity.