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目的:探讨生长分化因子GDF15(Growth Differentiation Factor 15)基因在卵巢上皮性癌组织中的表达及其与铂类耐药的相关性。方法:应用免疫组化、western blot、RT-PCR等方法对80例原发性卵巢癌组织和卵巢癌顺铂敏感/耐药株A2780和CP70、SKOV3和SKOV3/DDP中生长分化因子GDF15表达水平进行测定。结果:生长分化因子GDF15的表达强度与卵巢癌铂类耐药性显著相关。在卵巢癌顺铂耐药株CP70、SKOV3/DDP中GDF15表达水平较顺铂敏感株A2780、SKOV3明显增高。结论:GDF15表达水平与卵巢癌发生发展及铂类耐药相关,对于卵巢癌患者早期筛选、预测预后具有一定的临床指导价值。
Objective: To investigate the expression of Growth Differentiation Factor 15 (GDF15) gene in epithelial ovarian cancer and its relationship with platinum resistance. Methods: The expression of GDF15 in 80 cases of primary ovarian cancer and cisplatin sensitive / resistant strains A2780 and CP70, SKOV3 and SKOV3 / DDP of 80 cases of primary ovarian cancer was studied by immunohistochemistry, western blot and RT-PCR. Measurement was performed. Results: The expression intensity of GDF15 was significantly associated with platinum resistance in ovarian cancer. The expression level of GDF15 in cisplatin-resistant ovarian cancer cells CP70 and SKOV3 / DDP was significantly higher than that of cisplatin sensitive strains A2780 and SKOV3. Conclusion: The expression level of GDF15 is correlated with the occurrence and development of ovarian cancer and the drug resistance of platinum. It has certain clinical value for early screening and prognosis of patients with ovarian cancer.