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目的:观察大鼠癫发作后海马内神经元与星形胶质细胞反应变化的时空效应及相互关系。方法:以红藻氨酸诱发的大鼠复杂部分性癫发作为模型,利用免疫组织化学法,在原位显示癫发作后153、0、60、901、20、180 min 6个时间点海马神经元Fos蛋白及星形胶质细胞内胶质原纤维酸性蛋白(GFAP)的表达变化、相互关系及分布规律。结果:致后15 min海马内GFAP表达开始增多,60 min达高峰。Fos阳性神经元在癫诱发后30 min开始出现,120 min达高峰。海马内GFAP阳性细胞与Fos阳性神经元分布规律基本一致。结论:在癫病理状态下,海马内星形胶质细胞的反应略早于神经元,两者之间分布呈平行关系,它们之间可能存在着复杂的信息通讯,以复合体的形式共同对各种病理生理刺激作出反应。
OBJECTIVE: To observe the spatio-temporal effects of astrocytes in hippocampus and their correlation after epileptic seizures in rats. Methods: Kainic acid-induced rat partial complex epilepsy as a model, using immunohistochemistry in situ display of epilepsy 153,0,60,901,20,180 min at 6 time points after the hippocampal nerve Meta-Fos protein and astrocyte glial fibrillary acidic protein (GFAP) expression changes, correlation and distribution. Results: The expression of GFAP began to increase in hippocampus 15 min after reaching the peak at 60 min. Fos positive neurons began to appear 30 minutes after the onset of epilepsy, reached the peak at 120 minutes. The distribution of GFAP positive cells and Fos positive neurons in hippocampus were basically the same. CONCLUSION: In the epileptic state, the response of astrocytes in hippocampus is slightly earlier than that of neurons. The distribution of them is in a parallel relationship. There may be complex information and communication between them in the form of complex A variety of pathophysiological stimuli to respond.