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目的探索根据体重变化监测肝硬化诱导过程中大鼠对硫代乙酰胺 (thioacetamide,TAA)反应的个体差异 ,以提高肝硬化形成率和质量。方法雄性Wistar大鼠 4 6只 (2 0 0～ 2 30 g) ,随机分为 3组。 1组 (2 0只 )以 0 0 3% (w/v)TAA作为饮用水 ,共 12周。 2组 (2 0只 )以 0 0 3%TAA作为初始浓度 ,然后根据每周体重变化调整饮用水中TAA浓度。 3组 (6只 )给予饮用水 ,作为对照组。结果 1组大鼠病死率为 30 % (6 / 2 0 ) ,肝硬化形成率仅 4 5 % (9/ 2 0 )。 2组病死率为 0 ,肝硬化形成率为 90 % (18/ 2 0 )。结论肝硬化诱导过程中大鼠对TAA反应的个体差异可根据每周体重变化加以监测。该法可降低大鼠病死率至 0 ,同时显著提高肝硬化形成率和质量。
Objective To explore the individual differences in response to thioacetamide (TAA) in rats during liver cirrhosis induction according to changes in body weight to improve the rate and quality of liver cirrhosis. Methods Sixty-six male Wistar rats (200 ~ 230 g) were randomly divided into three groups. Group 1 (20 rats) received 0 3% (w / v) TAA as drinking water for 12 weeks. Group 2 (20) with TA 0 as the initial concentration, and then adjust the concentration of TAA in drinking water according to the weekly body weight changes. Three groups (6) were given drinking water as a control group. Results The mortality of rats in group 1 was 30% (6/20), and the incidence of cirrhosis was only 45% (9/20). Two groups of fatality rate was 0, the incidence of cirrhosis was 90% (18/20). Conclusion The individual differences in TAA response during cirrhosis induction in rats may be monitored on the basis of weekly body weight changes. The method can reduce the mortality in rats to 0, while significantly improving the rate and quality of liver cirrhosis.