论文部分内容阅读
目的:基于防己传统临床用药多为水煎煮,对防己水煎液中总生物碱进行富集,研究其对牛Ⅱ型胶原诱导大鼠关节炎模型(collagen-induced arthritis,CIA)的影响,区别以往对其醇提物的研究,为进一步探索防己水溶性大极性生物碱的抗风湿作用提供依据。方法:采用732型阳离子树脂富集防己水煎液中总生物碱。通过足底、尾部、背部皮内注射牛Ⅱ型胶原乳剂,7 d后再加强注射1次,建立CIA大鼠模型。设正常组、模型组、防己水煎液中总生物碱低、中、高剂量组(0.162,0.324,0.486 g·kg-1)、雷公藤多苷组(1.5 mg·kg-1)和地塞米松组(10.8 mg·kg-1)分别ig给药,连续给药21 d。观察不同剂量对CIA大鼠关节炎指数、足跖肿胀度的影响,同时测定血清中白介素(IL)-1,IL-1β,IL-6和肿瘤坏死因子-α(TNF-α)水平,以及观察踝关节组织病理变化。结果:与正常组比较,模型组关节炎指数和足跖肿胀度显著升高,血清IL-1,IL-1β,IL-6和TNF-α的水平显著升高(P<0.01);与模型组比较,防己水煎液中总生物碱高、中、低剂量组能够降低关节炎指数和足跖肿胀度,明显降低血清中IL-1,IL-1β,IL-6和TNF-α的水平(P<0.01),并且减轻滑膜组织的病理损伤。结论:防己水煎液总生物碱对于类风湿关节炎有一定的治疗作用,可明显降低血清中炎性因子水平。
OBJECTIVE: To study the effects of traditional alkaloids on the collagen-induced arthritis (CIA) induced by bovine type Ⅱ collagen in rats. Differences between the previous study of its alcohol extract, to further explore the anti-rheumatoid water-soluble polar alkaloids provide the basis. Methods: The total alkaloids in the decoction of Rhizoma Dioscoreae were enriched by 732 cationic resin. The bovine type Ⅱ collagen emulsion was injected intradermally through the soles of feet, tail and back, and then injected once more after 7 days to establish CIA rat model. The concentrations of total alkaloids in the normal group, model group and decoction of Rhizoma Dioscoreae of Rhizoma et Radix were determined by low, medium and high dose groups (0.162,0.324,0.486 g · kg-1), tripterygium glycosides group (1.5 mg · kg-1) The rats in the group of dexamethasone (10.8 mg · kg-1) were given ig administration for 21 days. The effects of different doses on arthritis index and plantar swelling in CIA rats were observed. The levels of IL-1, IL-1β, IL-6 and TNF-α in serum were also measured. Observing the pathological changes of ankle tissue. Results: Compared with the normal group, the arthritis index and paw edema significantly increased and the levels of IL-1, IL-1β, IL-6 and TNF-α in the model group were significantly increased (P <0.01) Compared with the control group, the total alkaloids in the decoction of Rhizoma Dioscoreae, Rhizoma Anemarrhenae and Rhizoma et Radix were significantly lower (P <0.01), and reduce the pathological damage of synovial tissue. Conclusion: The total alkaloids of Radix decoction have a certain therapeutic effect on rheumatoid arthritis, and can significantly reduce the level of inflammatory cytokines in serum.