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目的 从一对高低转移表型有差异的人肺巨细胞癌细胞株中分离并鉴定人类肿瘤转移抑制基因或具有转移抑制活性的核苷酸序列 ,为探讨肿瘤转移发生的分子生物学机制奠定基础。方法 使用抑制消减杂交技术 ,从一对同一亲本、转移表型不同的人肺巨细胞癌细胞株中分离转移抑制相关基因或核苷酸片段。结果 获得 5个在低转移肺巨细胞癌中高表达的、均与已知的人类基因片段有很高同源性的核苷酸片段。结论 利用抑制消减杂交技术 ,从一对同源的高低转移表型差异的细胞株中获得了 5条可能与肿瘤转移抑制相关的人cDNA序列 ,它们可能在维持肿瘤细胞自身稳定和防止肿瘤转移中起重要作用。
Objective To isolate and identify human tumor metastasis suppressor genes or nucleotide sequences with metastasis inhibitory activity from human lung giant cell carcinoma cell lines with different phenotypes of high and low metastasis, and to lay a foundation for exploring the molecular biological mechanism of tumor metastasis. . METHODS: Suppression subtractive hybridization was used to isolate metastasis-suppressing related genes or nucleotide fragments from a pair of human giant cell carcinoma cell lines with the same parent and different metastatic phenotypes. Results Five nucleotide fragments that were highly expressed in low metastatic lung giant cell carcinoma and were highly homologous to known human gene fragments were obtained. CONCLUSIONS: Using suppression subtractive hybridization, five human cDNA sequences that may be involved in tumor metastasis suppression were obtained from a homogenous cell line with high and low metastatic phenotypic differences, which may be in maintaining tumor cell self-stability and preventing tumor metastasis. makes an important impact.