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目的:探讨银杏内酯B对过氧化氢(H_2O_2)诱导的星形胶质细胞损伤的保护作用及可能机制。方法:星形胶质细胞传代培养,分为阴性对照组(以正常培养液培养),氧化损伤组(100μmol·L~(-1)的H_2O_2作用12 h),银杏内酯B低剂量组(1×10~(-6) mol·L~(-1)银杏内酯B孵育24 h后,加入H_2O_2作用12 h)和银杏内酯B高剂量组(1×10~(-4) mol·L~(-1)银杏内酯B孵育24 h后,加入H_2O_2作用12 h),MTT比色法检测细胞存活率,流式细胞仪检测细胞活性氧(ROS)水平,分光光度计检测上清液中超氧化物歧化酶(SOD)、如谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)的含量。结果:银杏内酯B能抑制氧化损伤引起的细胞活性的下降,降低星形胶质细胞内ROS的生成,促进SOD、GSH-Px水平的升高及MDA水平的下降。结论:银杏内酯B通过提高细胞内SOD、GSH-Px含量,降低细胞内MDA含量发挥其较强的抗氧化作用,从而为其用于治疗神经系统疾病提供可靠的实验依据。
Objective: To investigate the protective effect of ginkgolide B on hydrogen peroxide (H_2O_2) -induced astrocyte injury and its possible mechanism. Methods: The astrocytes were subcultured and divided into negative control group (cultured in normal culture medium), oxidative injury group (H 2 O 2 of 100μmol·L -1 for 12 h), Ginkgolide B low dose group After incubated with 1 × 10 ~ (-6) mol·L -1 Ginkgolide B for 24 h, H 2 O 2 was added for 12 h and Ginkgolide B high dose (1 × 10 -4 mol·L -1) L-ginkolide B was incubated for 24 h, H 2 O 2 was added for 12 h, cell viability was measured by MTT colorimetric assay, reactive oxygen species (ROS) levels were detected by flow cytometry, supernatant was detected by spectrophotometer Fluid superoxide dismutase (SOD), such as glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) content. Results: Ginkgolide B could inhibit the decrease of cell viability induced by oxidative damage, decrease the production of ROS in astrocytes, increase the level of SOD, GSH-Px and decrease the level of MDA. CONCLUSION: Ginkgolide B can exert its strong antioxidative effect by increasing intracellular SOD, GSH-Px content and decreasing intracellular MDA content, thus providing a reliable experimental basis for its application in the treatment of nervous system diseases.