用酵母产生的基因工程乙型肝炎疫苗已成功地用于动物试验。酵母合成HBsAg颗粒的步骤如下:(1)修饰HBsAg编码区,除去有碍表达的5′-未转译序列;(2)分离并使用几种新的酵母启动子,以有效地启动酵母中的 Genetically engineered hepatitis B vaccines produced using yeast have been successfully used in animal experiments. The steps of yeast synthesis of HBsAg particles are as follows: (1) modification of the HBsAg coding region to remove un-translated 5’- untranslated sequences; (2) isolation and use of several new yeast promoters to efficiently prime yeast