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柯萨奇Ｂ３非亲心肌株病毒（ＣＶＢ３ｏ）是否引起心肌炎还缺乏直接的实验依据。我们采用柯萨奇Ｂ３亲心肌株（ＣＶＢ３ｍ）和ＣＶＢ３ｏ感染鼠培养心肌细胞，对两株病毒感染细胞的特性进行了比较，结果表明，ＣＶＢ３ｏ与ＣＶＢ３ｍ在乳鼠心肌细胞培养液中的滴度，１２小时和２４小时无明显差别，４８小时后ＣＶＢ３ｍ的滴度变化不大，而ＣＶＢ３ｏ有所下降；两株病毒均能使鼠心肌细胞死亡，但随着时间的延长ＣＶＢ３ｍ感染后心肌细胞死亡率明显高于ＣＶＢ３ｏ；ＣＶＢ３ｏ不能阻断ＣＶＢ３ｍ对心肌细胞的感染。因此我们认为，ＣＶＢ３ｏ能感染心肌细胞，但其程度低于ＣＶＢ３ｍ。
Coxsackie B3 non-parental virus (CVB3o) is caused by myocarditis is still lack of direct experimental evidence. We use the Coxsackie B3 myocardial cell strain (CVB3m) and CVB3o infected rat cultured cardiomyocytes, the characteristics of the two virus-infected cells were compared, the results showed that CVB3o and CVB3m in neonatal rat cardiomyocyte culture fluid titer, 12 hours and 24 hours no significant difference, 48 hours after CVB3m little change in the titer, and CVB3o decreased; two viruses can cause cardiac myocyte death, but with the passage of time CVB3m infection after myocardial cell death Significantly higher than CVB3o; CVB3o can not block the CVB3m on myocardial cell infection. Therefore, we believe that CVB3o can infect myocytes but to a lesser degree than CVB3m.