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Studies in recent years have demonstrated that HPV16 (human papilloma-virus type16) E6 and E7 gene expression plays an important role in the pathogenesis and the maintenance of malignant phenotype of anogenital cancer, particularly of cervical cancer. It was proved that anti-HPV16 E7-ribozyme was able to specifically cleave the E7 mRNA fragment in vitro. We studied the expression of ribozyme in eucaryotic cells by means of
Studies in recent years have the HPV16 (human papilloma-virus type16) E6 and E7 gene expression plays an important role in the pathogenesis and the maintenance of malignant phenotype of anogenital cancer, particularly the cervical cancer. It was proved that anti-HPV16 E7 -ribozyme was able to specifically cleave the E7 mRNA fragment in vitro. We studied the expression of ribozyme in eucaryotic cells by means of