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为探究壳聚糖诱导促进红松(Pinus koraiensis)多酚合成的生理调控机制,以红松幼苗为实验材料,在DCR培养基中添加不同浓度的壳聚糖,诱导8天后测定多酚和原花青素的含量,筛选有利于多酚积累的最佳的壳聚糖浓度。随后测定最佳浓度壳聚糖诱导下红松幼苗中多酚物质积累量、防御酶活性和多酚合成途径关键酶活性随时间的变化。结果显示:50–200mg·L–1壳聚糖可以有效地提高多酚和原花青素的积累量。壳聚糖浓度为100 mg·L–1时诱导效果最佳,多酚积累量可以达到(9.91±0.68)mg·g–1鲜质量,是对照组的1.64倍;原花青素积累量可以达到(2.52±0.11)mg·g–1鲜质量,是对照组的1.53倍。100mg·L–1壳聚糖诱导下红松幼苗防御相关酶(超氧化物歧化酶、过氧化物酶、过氧化氢酶)和多酚合成关键酶(苯丙氨酸转氨酶、肉桂酸4-羟化酶)迅速做出响应,活性显著高于对照组。壳聚糖能够显著地激活红松幼苗的防御反应和苯丙烷代谢途径,从而促进抗氧化物质多酚的合成与积累,有利于提高红松幼苗的抵抗力。
In order to explore the mechanism of chitosan-induced physiological regulation of polyphenol synthesis in Pinus koraiensis, different concentrations of chitosan were added to the DCR medium with the seedlings of Pinus koraiensis as test materials. After 8 days of induction, polyphenols and proanthocyanidins The optimal chitosan concentration for polyphenol accumulation was screened. The accumulation of polyphenols, the activities of defense enzymes and the key enzyme activities of polyphenol synthesis pathway under the optimal concentration of chitosan were determined. The results showed that: 50-200mg · L-1 chitosan can effectively increase the accumulation of polyphenols and proanthocyanidins. The optimal concentration of chitosan was 100 mg · L-1, and the accumulation of polyphenols could reach (9.91 ± 0.68) mg · g-1 fresh weight, which was 1.64 times that of the control group. The accumulation of proanthocyanidins reached 2.52 ± 0.11) mg · g-1 fresh weight, which is 1.53 times that of the control group. Under the induction of 100 mg · L-1 chitosan, the defense-related enzymes (superoxide dismutase, peroxidase and catalase) and the key enzyme of polyphenols synthesis (phenylalanine aminotransferase, cinnamic acid 4- Hydroxylase) to respond quickly, the activity was significantly higher than the control group. Chitosan can significantly activate the defense reaction and phenylpropanoid metabolic pathway of Korean pine seedlings to promote the synthesis and accumulation of antioxidant polyphenols, which is beneficial to improve the resistance of Korean pine seedlings.