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目的建立大鼠肺纤维化模型,并对其进行评价。方法经气管内喷雾注入博莱霉素(Bleomycin,BLM)致大鼠肺纤维化,于第1、2、3、4周处死大鼠,观察大鼠给药后日常状况和体重变化,测定羟脯氨酸(L-hydroxyproline-trans-4-hydroxy-L-proline,HYP)含量、肺重,计算肺系数,取肺组织制备病理切片,进行HE、Masson染色后,评价肺组织形态学变化。结果给药4周后,模型组大鼠肺部产生了明显肺纤维化症状,肺系数及HYP含量均明显高于对照组,肺泡间隔增厚,纤维化区可见大量蓝色胶原纤维沉积。结论大鼠气管内喷雾注入BLM可成功制备肺纤维化模型,且纤维化分布较均匀。
Objective To establish a rat model of pulmonary fibrosis and evaluate its effects. Methods Pulmonary fibrosis was induced by intratracheal instillation of bleomycin (BLM) in rats. The rats were sacrificed at 1, 2, 3 and 4 weeks. The daily and post-administration rats’ L-hydroxyproline-L-hydroxyproline (L-hydroxyproline-4-hydroxy-L-proline) content and lung weight, calculate the lung coefficient, take the lung tissue to prepare the pathological section, Results After 4 weeks of administration, the lungs of the model group developed obvious symptoms of pulmonary fibrosis. The pulmonary coefficient and HYP content in the model group were significantly higher than those in the control group. The alveolar septum was thickened and a large amount of blue collagen fibers were seen in the fibrosis area. Conclusion Intratracheal instillation of BLM into rats can successfully prepare pulmonary fibrosis model and the fibrosis is more evenly distributed.