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目的 研究端粒酶在各种甲状腺肿瘤中的活性状态。方法 用改良的以PCR为基础的端粒重复序列扩增法 (TRAP PCR) ,在定性及定量水平上检测了 10 0例良恶性甲状腺组织标本及 15例甲状腺癌细针穿刺标本的端粒酶活性。结果 甲状腺癌组织的端粒酶阳性率 (83 8% ,31/ 37例 )明显高于良性甲状腺病变 (2 1 2 % ,7/ 33例 )及病变旁正常甲状腺组织 (13 3% ,4/ 30例 ) ;15例甲状腺癌的细针穿刺标本也均呈阳性表达 ,与相应的组织标本相符。定量结果显示甲状腺癌组端粒酶活性明显高于良性病变及正常组织 ;髓样癌及间变癌的活性又高于乳头状癌。结论 端粒酶活性为恶性甲状腺肿瘤的一个敏感标志物。TRAP PCR定量分析对甲状腺肿瘤的鉴别诊断具有重要意义
Objective To study the activity of telomerase in various thyroid tumors. Methods Tested PCR-based telomeric repeat amplification (TRAP PCR) method was used to detect the telomerase activity in 100 specimens of benign and malignant thyroid tissue and 15 specimens of thyroid cancer with fine needle aspiration on qualitative and quantitative basis active. Results The positive rate of telomerase (83.8%, 31/37 cases) in thyroid cancer was significantly higher than that in benign thyroid lesions (21.2%, 7/33 cases) and adjacent normal thyroid tissues (13.3%, 4 / 30 cases); fine needle aspiration specimens of 15 cases of thyroid cancer were also positive expression, consistent with the corresponding tissue samples. Quantitative results showed that the telomerase activity of thyroid cancer was significantly higher than that of benign lesions and normal tissues; the activity of medullary carcinoma and metaplastic carcinoma was higher than that of papillary carcinoma. Conclusion Telomerase activity is a sensitive marker of malignant thyroid neoplasm. TRAP PCR quantitative analysis of thyroid tumors in the differential diagnosis is of great significance