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目的 研究端粒酶活性在大肠癌、癌旁组织、正常大肠黏膜及大肠良性病变组织中的表达情况 ,探讨其作为大肠癌生物学标志物的可能性。方法 PCR TRAP ELISA法检测 30例大肠癌、癌旁组织及正常黏膜和 2 0例大肠腺瘤性息肉组织中端粒酶活性水平。结果 (1 ) 30例大肠癌组织、癌旁组织、正常大肠组织及 2 0例大肠腺瘤性息肉组织中端粒酶活性分别为83 33% ,1 3 33% ,3 33%和 1 0 0 0 % ,癌组织中端粒酶活性明显高于其他组织 (P <0 0 5 ) ;(2 )伴淋巴结转移大肠癌端粒酶阳性表达率为 80 % (8/ 1 0 ) ,无淋巴结转移者为 0 % (0 / 2 0 ) ,二者比较有非常显著性差异 (P <0 0 0 1 ) ;(3)大肠癌组织端粒酶活性随组织学分级、临床分期而呈逐渐增高趋势 ,但统计学处理差异无显著性 (P >0 0 5 )。结论 端粒酶活性的检测可作为大肠癌诊断的指标之一 ,对判断大肠癌的预后具有重要参考价值。
Objective To study the expression of telomerase activity in colorectal cancer tissues, paracancerous tissues, normal colorectal mucosa and benign colorectal lesions and to explore its potential as a biomarker of colorectal cancer. Methods The telomerase activity in 30 cases of colorectal cancer, paracancerous tissues and normal mucosa and 20 cases of colorectal adenoma was detected by PCR TRAP ELISA. Results (1) The telomerase activity in 30 cases of colorectal cancer tissues, paracancerous tissues, normal colorectal tissues and 20 cases of colorectal adenomatous polyps were 83 33%, 13 33%, 33 33% and 100% (P <0.05); (2) The positive rate of telomerase in colorectal cancer with lymph node metastasis was 80% (8/10), no lymph node metastasis (0/20), there was a significant difference between the two groups (P <0.01). (3) The telomerase activity in colorectal cancer tissues increased gradually with the histological grade and clinical stage , But no statistically significant difference (P> 0.05). Conclusion The detection of telomerase activity can be used as one of the indicators of the diagnosis of colorectal cancer, which has an important reference value for judging the prognosis of colorectal cancer.