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观察抗氧化剂２（３）┐叔丁基┐４┐羟基茴香醚（ＢＨＡ）对ＨＢＶ转基因小鼠肝肿瘤的防护作用。方法２６只ＨＢＶ转基因和２４只非转基因小鼠，用生化法测定肝脏醌还原酶（ＱＲ）和谷胱甘肽Ｓ┐转移酶（ＧＳＴｓ）活性、丙二醛（ＭＤＡ）含量和免疫组化法研究肝肿瘤ＰＣＮＡ表达。结果转基因小鼠ＢＨＡ组肝癌发生率０，显著低于普饲组４２％（５／１２例）。ＢＨＡ诱导肝脏ＱＲ和ＧＳＴ活性升高３～７倍，降低ＭＤＡ。ＢＨＡ组肝腺瘤ＰＣＮＡ标记指数３．４９±２．４２％，显著低于普饲组（５．４８±２．２９％）。结论ＢＨＡ长期应用有效延缓了ＨＢＶ转基因小鼠肝肿瘤的发生发展进程。
To observe the protective effect of antioxidant 2(3)-tert-butyl-4-hydroxyanisole (BHA) on hepatic tumors in HBV transgenic mice. Methods 26 HBV transgenes and 24 non-transgenic mice were used to determine the activity of liver chymase reductase (QR) and glutathione S glutathione transferase (GSTs), malondialdehyde (MDA) content and immunohistochemical method. Study liver tumor PCNA expression. Results The incidence of liver cancer in the BHA group was 0, which was significantly lower than that of the normal group (42%, 5/12). BHA induced a 3 to 7-fold increase in liver QR and GST activity and decreased MDA. The PCNA labeling index of hepatic adenoma in BHA group was 3.49±2.42%, which was significantly lower than that in the fed group (5.48±2.29%). Conclusion The long-term application of BHA can effectively delay the development of liver tumors in HBV transgenic mice.