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目的了解福州市服务行业健康人员携带沙门菌情况、血清型分布及耐药性分析,为防控沙门菌感染及食物中毒提供依据。方法肛拭采集1993年-2015年从业人员粪便标本,应用沙门菌选择性增菌液培养并经选择性培养基分离,挑取可疑菌落至KIA及L-M琼脂初筛,最后经血清学试验及系统生化检测确认。结果 1993年-2015年共检出474株沙门菌,47个血清型、3个单相变种和1个无鞭毛抗原。绝大多数菌株血清型集中于A~F群内(99.37%),A群~F群内有44个血清型(93.62%),以C1群血清型为主,其次为C2和B群;血清型检出率占前5位的分别是德尔卑、肠炎、鼠伤寒、韦太夫雷登和伦敦血清型;绝大多数菌株对10种抗生素耐药率较低;从业人员沙门菌携带率为0.46%~1.31%;第三季度是沙门菌检出高峰期。结论加强服务从业人员沙门菌监测工作,对预防沙门菌感染和食物中毒的发生有着非常重要的意义。
Objective To understand the situation of salmonella carrying serotypes, serotype distribution and drug resistance of health workers in service industry in Fuzhou, and to provide basis for the prevention and control of Salmonella infection and food poisoning. Methods Anal swabs were collected from the stool samples of practitioners from 1993 to 2015. The stool samples were cultured in selective enrichment broth of Salmonella and separated by selective medium. Suspicious colonies were picked to primary screening of KIA and LM agar. Finally, serological tests and system Biochemical test confirmed. Results A total of 474 Salmonella, 47 serotypes, three single-phase variants and one non-flagella antigen were detected in 1993-2015. The serotypes of most strains were concentrated in A to F groups (99.37%), 44 serotypes (93.62%) in group A to group F, the majority of which were serogroups C1 and C2 and B, followed by serum The detection rate of the top five were Delvill, enteritis, typhoid, Wyeth and London serotypes; the majority of isolates were resistant to 10 antibiotics; the carrying rate of salmonella was 0.46 % ~ 1.31%; the third quarter is the peak period of detection of Salmonella. Conclusion Strengthening the monitoring of Salmonella among service workers is of great significance in preventing the occurrence of Salmonella infection and food poisoning.