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目的：探讨微囊藻毒素-LR对小鼠肝、肾和睾丸组织蛋白质的氧化损伤程度。方法：用3.0、6.0和12.0μg/kg3种不同浓度的微囊藻毒素-LR对小鼠进行腹腔注射染毒（n=5）,染毒时间为7d,每天1次,并设阴性对照组。用2,4-二硝基苯肼比色法测定3种组织的蛋白质羰基含量。结果：随着微囊藻毒素-LR剂量的升高,小鼠肝、肾和睾丸的蛋白质羰基含量升高（F分别为44.631、21.975和23.962,P均〈0.001）。3.0μg/kg染毒组肝、肾蛋白质羰基含量[（3.72±0.23）和（3.99±0.48）mmol/kg]高于阴性对照组[（2.90±0.22）和（3.27±0.23）mmol/kg]（P均〈0.05）。3.0μg/kg染毒组睾丸蛋白质羰基含量[（1.91±0.25）mmol/kg]与阴性对照组[（1.69±0.27）mmol/kg]相比,差异无统计学意义（P〉0.05）。6.0μg/kg染毒组肝、肾和睾丸蛋白质羰基含量[（4.55±0.45）、（4.44±0.53）和（2.53±0.24）mmol/kg]和12.0μg/kg染毒组肝、肾和睾丸蛋白质羰基含量[（5.62±0.55）、（5.67±0.60）和（3.15±0.41）mmol/kg]均高于阴性对照组（P均〈0.01）。结论：微囊藻毒素-LR对小鼠肝、肾和睾丸组织的蛋白质有氧化损伤作用。“,”Aim：To expore the degree of protein oxidative damage on liver, kindey and testicle of mice induced by microcystin-LR.Methods：A total of 20 mice were intraperitoneally administered microcystin-LR at doses of 0,3.0, 6.0 or 12.0 μg/kg body weight every day for 7 days.The protein carbonyl content in liver,kidney and testicle tissue was measured using spectrophotometric DNPH assay to reflect the degree of protein oxidative damage.Results：With the increase of microcystin-LR concentration protein carbonyl content in three kinds of tissue also increased（F was 44.631, 21.975 and 23.962,P0.001）. Protein carbonyl contents of liver and kidney in 3.0 μg/kg microcystin-LR exposure group [（3.72±0.23） and （3.99±0.48） mmol/kg] were higher than those of control group [（2.90±0.22） and （3.27±0.23） mmol/kg] （P0.05）. There was no significant difference in protein carbonyl content of testicle between 3.0 μg/kg microcystin-LR exposure group [（1.91±0.25） mmol/kg] and control group [（1.69±0.27） mmol/kg] （P0.05）. Protein carbonyl content of liver, kidney,and testicle in 6.0 μg/kg microcystin-LR exposure group [（4.55±0.45）, （4.44±0.53）, （2.53±0.24） mmol/kg] and 12.0 μg/kg microcystin-LR exposure group [（5.62±0.55）, （5.67±0.60）,（3.15±0.41） mmol/kg] were higher than those of control group（P0.01）.Conclusion： Microcystin-LR could induce protein oxidative damage of liver, kidney and testicle in mice.