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为研究产单核细胞李斯特菌 (LM )感染对小鼠胸腺细胞凋亡的诱导作用及胸腺细胞凋亡过程中的基因调控 ,小鼠经尾静脉注射LM后 ,以DNA琼脂糖凝胶电泳和流式细胞仪 (FCM )检测细胞凋亡及凋亡细胞的基因产物表达水平。结果表明LM能诱导小鼠胸腺细胞凋亡 ,琼脂糖凝胶电泳分析显示胸腺细胞出现典型的DNA“梯状带” ,FCM分析显示特征性的凋亡峰。胸腺细胞凋亡于LM (5× 10 5CFU )感染后 8h出现 ,48h达高峰。胸腺细胞凋亡百分率随LM感染剂量增加而增高。LM诱导小鼠胸腺细胞凋亡中p5 3、Bax及c myc基因表达产物明显增加 ,而Bcl 2基因表达产物水平无明显改变。提示LM以时间和剂量依赖方式诱导小鼠胸腺细胞凋亡 ,p5 3、Bax及c myc基因在LM诱导小鼠胸腺细胞凋亡的基因调控中起重要作用。
To study the induction of Listeria monocytogenes (LM) infection on thymocyte apoptosis in mice and the gene regulation during thymocyte apoptosis, mice were injected LM with caudal vein and then subjected to DNA agarose gel electrophoresis And flow cytometry (FCM) were used to detect the apoptosis and expression of gene products in apoptotic cells. The results showed that LM can induce thymocyte apoptosis in mice. The typical DNA “ladder” appeared in thymocytes by agarose gel electrophoresis. FCM analysis showed the characteristic apoptotic peak. Thymocyte apoptosis appeared at 8h after LM (5 × 10 5 CFU) infection and peaked at 48h. The percentage of thymus apoptosis increased with the increasing dose of LM infection. LM induced thymocyte apoptosis in mice p5 3, Bax and c myc gene expression products increased significantly, while the Bcl 2 gene expression product levels did not change significantly. These results suggest that LM induces apoptosis in mouse thymocytes in a time and dose dependent manner. The expression of p5 3, Bax and c myc plays an important role in gene regulation of LM induced thymocyte apoptosis in mice.