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Objective:To observethedifferenceof geneexpressionsof braintissuesduringapoplecticepisodesandthose of normalbraininWistarratsinorderto studythepathologicalmechanismof apoplexy.Methods:A ratmodelof hyper-tensionwas establishedwiththeadministrationof coldstimulusandhighsaltintakeas theenvironmentalriskfactors.Apoplexyoccurredin theratsbecauseof hypertension.Suppressionsubtractivehybridization(SSH)was usedto identify andanalyzethedifferentialgenesspecificallyexpressedincerebraltissuesof stokegroupandcontrolrats.Re sults:A to-talof226genesoutof the228wereusableandanalyzed.Theaveragelengthof the226geneswas(286.6±120.3)bp with a rangefrom50bp to619bp.And126clonesoutof the226showeda sequencewithsignificantidentityto theknown genes;78clonesdemonstratedhomogenoussequencesto theexistingESTsof dbEST,butno oneof the78showedse-quencewithidentityto thatof knowngenes;andremaining22werenoveltransrciptsexhibitingno similarity to any knownsequences.Allthecloneswhichwerehighlyhomogenousto theknowngeneswerecategorizedon thebasisof their function.It was foundthat26.5%of themitochodrialgenesin braintissuesunderwentchangesafterapoplexyandthe changesshoweda twofoldrelationshipof causeandeffect.Conclusion:Environmentalfactorsareableto inducechanges of geneexpression,whichmayincreasethesensitivityto apoplecticstroke.
Objective: To observethedifferenceof geneexpressionsof braintissuesduringapoplecticepisodesandthose of normalbraininWistarratsinorderto studythepathologicalmechanismof apoplexy.Methods: A ratmodelof hyper-tensionwas establishedwiththeadministrationof coldstimulusandhighsaltintakeas theenvironmentalriskfactors.Apoplexyoccurredin theratsbecauseof hypertension.Suppressionsubtractivehybridization (SSH) was usedto identify andanalyzethedifferentialgenesspecificallyexpressedincerebraltissuesof stokegroupandcontrolrats.Re sults: A to-talof226genesoutof the228wereusableandanalyzed.Theaveragelengthof the226geneswas (286.6 ± 120.3) bp with a range from 50 bp to 619 bp. And 126 clones out of the 226 showeda sequence with unique identity to the known genes; 78 clones demonstrated homogenous sequence in the imposing ESTs of dbEST, but no one of the 78 shy implies-quence withidentity to that of known gens; and remaining of 22 de novo transversals in the identification of any known sequences. All of these clones are highlyhomogenousto the known genes erecategorizedon thebasisof theirfunction.Itwas foundthat26.5% of themitochodrialgenesin braintissuesunderwentchangesafterapoplexyandthechangesshoweda twofoldrelationshipof causeandeffect.Conclusion: Environmentalfactorsareableto inducers of geneexpression, whichmayincreasethesensitivityto apoplecticstroke.