Micron-sized, monodisperse, superparamagnetic, luminescent composite poly(glycidyl methacrylate) (PGMA) microspheres with functional amino-groups were successfully synthesized in this study. The process of preparation was as follows: preparation of monodisperse poly(glycidyl methacrylate) mi- crospheres by dispersion polymerization method; modification of poly(glycidyl methacrylate) micro- spheres with ethylene diamine to form amino-groups; impregnation of iron ions (Fe2+ and Fe3+) inside the microspheres and subsequently precipitating them with ammonium hydroxide to form magnetite (Fe3O4) nanoparticles within the polymer microspheres; infusion of CdSe/CdS core-shell quantum dots (QDs) into magnetic polymer microspheres. Scanning electron microscopy (SEM) was used to char- acterize surface morphology and size distribution of composite microspheres. The average size of mi- crospheres was 1.42 μm with a size variation of 3.8%. The composite microspheres were bright enough and easily observed using a conventional fluorescence microscope. The composite microspheres were easily separated from solution by magnetic decantation using a permanent magnet. The new multi- functional composite microspheres are promising to be used in a variety of bioanalytical assays in- volving luminescence detection and magnetic separation. Micron-sized, monodisperse, superparamagnetic, luminescent composite poly (glycidyl methacrylate) (PGMA) microspheres with functional amino-groups were successfully synthesized in this study. The process of preparation was as follows: preparation of monodisperse poly (glycidyl methacrylate) mi- crospheres by dispersion polymerization method; modification of poly (glycidyl methacrylate) micro-spheres with ethylene diamine to form amino-groups; impregnation of iron ions (Fe2 + and Fe3 +) inside the microspheres and subsequent precipitating them with ammonium hydroxide to form magnetite (Fe3O4) nanoparticles within the polymer microspheres; infusion of CdSe / CdS core-shell quantum dots (QDs) into magnetic polymer microspheres. Scanning electron microscopy (SEM) was used to char- acterize surface morphology and size distribution of composite microspheres. The average size of mi- The composite microspheres were bright enough and easily obs The new multifunctional composite microspheres are promising to be used in a variety of bioanalytical assays in- volving luminescence detection and magnetic separation.