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目的对绵萆薢中甾体类成分进行研究,为其在中医药及临床方面的研究提供依据。方法采用大孔吸附树脂、正相硅胶、反相ODS、Sephadex LH-20等柱色谱及制备型高效液相色谱等方法对其所含甾体类成分进行分离、纯化,并结合化合物的理化性质与波谱学数据鉴定结构。结果与结论从绵萆薢中分离鉴定了10个甾体,分别为薯蓣皂苷元(1,diosgenin)、薯蓣皂苷次级皂苷A(2,prosapogenin A of dioscin)、diosgenin 3-O-β-D-glucopyranosyl(1→3)-β-D-glucopyranoside(3)、薯蓣皂苷次级皂苷B(4,prosapogenin B of dioscin)、薯蓣皂苷(5,dioscin)、纤细皂苷(6,gracillin)、7-oxodioscin(7)、(3β,7β,25R)-spirost-5-ene-3,7-diol-3-O-β-D-glucopyranosyl-(1→3)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside(8)、orbiculatoside B(9)、borassoside B(10)。化合物8和10为从薯蓣属植物中首次分离得到,化合物3、4、7为从该种植物中首次分离得到。
OBJECTIVE: To study the steroid constituents in Gynura aurata and provide the basis for its research in traditional Chinese medicine and clinical research. Methods The macroporous resin, normal phase silica gel, reverse phase ODS, Sephadex LH-20 column chromatography and preparative high performance liquid chromatography were used to separate and purify the steroid components. The physicochemical properties Identification of structures with spectroscopic data. RESULTS AND CONCLUSION Ten sterols were isolated and identified from cotton mullet, including diosgenin, prosapogenin A of dioscin, diosgenin 3-O-β-D- glucopyranosyl (1 → 3) -β-D-glucopyranoside (3), prosapogenin B of dioscin, dioscin, gracillin, 7-oxodioscin (1 → 3) - [α-L-rhamnopyranosyl- (1 → (7 → 3) -spirost-5-ene-3,7-diol- 2)] - β-D-glucopyranoside (8), orbiculatoside B (9), borassoside B (10). Compounds 8 and 10 were isolated from the genus Dioscorea for the first time, and compounds 3, 4 and 7 were isolated from this plant for the first time.