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目的:研究苦荞麦总黄酮对于软脂酸诱导EA.hy926细胞PI3K合成的影响。方法:将体外培养的EA.hy926细胞分为正常组、模型组、苦荞麦总黄酮组及二甲双胍组。采用RT-PCR以及免疫细胞化学法分别测定各组细胞PI3K mRNA和蛋白的表达。结果:模型组细胞PI3K mRNA和蛋白表达与正常组相比明显下降(P<0.01)。苦荞麦总黄酮组与二甲双胍组细胞PI3K mRNA和蛋白表达与模型组相比明显增加(P<0.01)。治疗组间无明显差异。结论:苦荞麦总黄酮对于软脂酸诱导下EA.hy926细胞PI3K合成具有明显促进作用。
AIM: To investigate the effect of total flavonoids from buckwheat on PI3K synthesis induced by palmitate in EA.hy926 cells. Methods: EA.hy926 cells cultured in vitro were divided into normal group, model group, total flavonoids of buckwheat group and metformin group. RT-PCR and immunocytochemistry were used to determine the expression of PI3K mRNA and protein in each group. Results: The expression of PI3K mRNA and protein in the model group was significantly decreased compared with the normal group (P <0.01). Compared with the model group, PI3K mRNA and protein expression in the total flavonoids group and metformin group increased significantly (P <0.01). There was no significant difference between treatment groups. Conclusion: Total flavonoids from buckwheat can promote the PI3K synthesis of EA.hy926 cells induced by palmitate.