目的：研究测定乳酸钠注射液中Ｌ乳酸钠含量的方法。方法：以固定化Ｌ乳酸氧化酶与Ｈ２Ｏ２电极构成酶电极，研究了乳酸（盐）酶电极分析法的各种分析条件及参数，并与非水滴定法对比测定了乳酸钠注射液中Ｌ乳酸钠及Ｄ，Ｌ外消旋乳酸钠的含量。结果：供试乳酸钠注射液中Ｌ乳酸钠含量约占乳酸钠总量的１０％。结论：乳酸钠注射液属于不等量立体异构体药物。采用酶电极法测定乳酸钠注射液中的Ｌ乳酸钠含量具有专一性高、成本低、分析速度快等优点。 Objective: To study the method of determining sodium lactate content in sodium lactate injection. Methods: The immobilized L-lactate oxidase and H2O2 electrode constitute the enzyme electrode, the lactate (salt) enzyme electrode analysis of various analytical conditions and parameters, and compared with non-aqueous titration of sodium lactate L lactate And D, L racemic sodium lactate content. Results: The content of L-lactate in the sodium lactate injection was about 10% of the total amount of sodium lactate. Conclusion: Sodium lactate injection belongs to unequal amount of stereoisomer drug. The determination of L-lactate in sodium lactate injection by enzyme electrode has the advantages of high specificity, low cost and fast analysis.