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利用Ｆｕｒａ２技术和ＡＲＣＭＭＩＣ阳离子系统研究了粉防己碱对传代培养牛主动脉血管平滑肌细胞内游离钙的影响。粉防己碱（１～１００μｍｏｌ·Ｌ－１）不影响血管平滑肌细胞的静息钙，但在有胞外钙存在时，对高钾，５羟色胺，ＡＴＰ，血管紧张素Ｉ，去甲肾上腺素引起的胞内钙升高有抑制作用；在无胞外钙存在时，粉防己碱对苯肾上腺素引起的胞内钙升高也有一定的抑制作用。粉防己碱对高钾升高内钙的抑制作用呈剂量依赖性，ＩＣ５０值为９．２（９５％可信限：５．７～１４．９）μｍｏｌ·Ｌ－１。上述结果提示粉防己碱对血管平滑肌细胞的电压依赖性钙通道和受体调控性钙通道均有阻滞作用，对受体调控性钙通道阻滞作用主要表现为阻滞钙离子内流。
The effects of tetrandrine on intracellular free calcium in cultured aortic vascular smooth muscle cells of subcultures were studied using Fura2 technology and ARCMMIC cationic system. Tetrandrine (1 ~ 100μmol·L-1) did not affect the resting calcium of vascular smooth muscle cells, but in the presence of extracellular calcium, the effects of high potassium, serotonin, ATP, angiotensin I, norepinephrine Caused by intracellular calcium increased inhibition; in the absence of extracellular calcium, tetrandrine on phenylephrine-induced increase in intracellular calcium also have some inhibitory effect. Tetrandrine had a dose-dependent inhibitory effect on elevated intracellular calcium with an IC50 of 9.2 (95% confidence interval: 5.7-14.9) μmol·L-1. These results suggest that tetrandrine can block the voltage-dependent calcium channel and the receptor-regulated calcium channel of vascular smooth muscle cells, and block the receptor-regulated calcium channel mainly as blocking calcium influx.