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目的通过小鼠结肠腺癌恶病质模型实验探索传统中药提取物黄连素改善恶病质状态的作用及机制。方法将40只BALB/c小鼠随机分成5组,每组8只。即非荷瘤健康对照组、肿瘤恶病质组、黄连素低剂量干预组、黄连素中剂量干预组、黄连素高剂量干预组,使用小白鼠结肠腺癌细胞株CT-26皮下注射BALB/c小鼠,构建荷瘤小鼠肿瘤恶病质模型。黄连素低、中、高剂量干预组小鼠分别给予黄连素12.5 mg/(kg·d)、25 mg/(kg·d)、50mg/(kg·d)灌胃,连续15天。健康对照组、肿瘤恶病质组给予等量生理盐水灌胃。观察低、中、高剂量黄连素对恶病质小鼠的体质量、摄食量、饮水量以及血清中三酰甘油、血糖及总蛋白、白蛋白水平的影响,并比较各组肿瘤组织炎性细胞因子白细胞介素1(interleukin-1,IL-1)、IL-6、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)水平以及肝、肾组织形态。观察不同剂量黄连素纠正恶病质的疗效及对肝肾的保护作用。结果与肿瘤恶病质组比较,黄连素干预各组能抑制癌症恶病质小鼠体质量下降,增加小鼠的摄食量、饮水量(均P<0.05);可升高血中总蛋白、白蛋白、血糖浓度,降低血清中三酰甘油的异常升高(均P<0.05);可降低肿瘤组织中的IL-1、IL-6及TNF-α表达水平(均P<0.05);可改善肝、肾组织形态。结论黄连素能有效改善小鼠结肠腺癌恶病质状态,以黄连素中、高剂量组疗效更佳,其机制可能与其有效降低细胞因子(IL-1、IL-6及TNF-α)表达相关。
Objective To explore the mechanism and mechanism of the effect of traditional Chinese medicine berberine on cachexia in mouse model of cachexia of colonic adenocarcinoma. Methods Forty BALB / c mice were randomly divided into 5 groups with 8 mice in each group. Namely non-tumor-bearing healthy control group, tumor cachexia group, berberine low dose intervention group, berberine medium dose intervention group and berberine high dose intervention group. The mouse colon adenocarcinoma cell line CT-26 was subcutaneously injected with BALB / c To construct tumor cachexia model in mice with tumor. Berberine low, medium and high dose intervention groups were given berberine 12.5 mg / (kg · d), 25 mg / (kg · d), 50mg / (kg · d) intragastrically for 15 consecutive days. The healthy control group and the tumor cachexia group were given normal saline. The effects of low, medium and high doses of berberine on the body weight, food intake, water intake, serum triglyceride, blood glucose and total protein and albumin in low, medium and high dose mice were observed. The levels of inflammatory cytokines Interleukin-1 (IL-1), IL-6, tumor necrosis factor-α (TNF-α) and liver and kidney tissue morphology. To observe the effect of different doses of berberine to correct cachexia and its protective effect on liver and kidney. Results Compared with the tumor cachexia group, berberine intervention could inhibit the decrease of body weight of mice with cancer cachexia and increase the food intake and water intake of mice (all P <0.05); increase the total protein, albumin, blood glucose (P <0.05). It could decrease the expression of IL-1, IL-6 and TNF-α in the tumor tissue (all P <0.05), and improve the liver and kidney Organizational form. Conclusion Berberine can effectively improve the state of cachexia in mice with colonic adenocarcinoma. The effect of berberine in medium and high dose group is better than that of berberine, and its mechanism may be related to the decrease of the expression of IL-1, IL-6 and TNF-α.