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目的通过对毛竹中腐胺年动态变化的研究,为毛竹抗旱研究提供参考。方法反相液相色谱法测定毛竹不同组织中的腐胺。结果毛竹组织中腐胺含量呈现多样的变化,总的来说各组织中腐胺含量为竹叶>竹枝>竹鞭,大小年毛竹多胺的变化呈现出不同的趋势,小年毛竹竹叶7月时含量较高,为11.39μg/g,随着时间推移腐胺的含量逐渐下降,进入春笋大年后腐胺含量增加;而小年竹枝中7月腐胺含量较低,为4.99μg/g,进入出笋大年后也呈现增加的趋势;竹鞭的腐胺含量7月最小,为2.50μg/g,次年4月出现大幅下降,含量仅为1.09μg/g。结论探索多胺对竹子笋芽分化的影响具有一定意义,多胺能够提高竹子的抗旱性,而且竹子能吸收外源多胺。将多胺作为生长调节物质研究竹子抗旱机理也是未来研究的方向。
Objective To study the dynamic changes of putrescine in bamboo so as to provide a reference for the research on drought resistance of bamboo. Methods RP-HPLC was used to determine putrescine in different tissues of Phyllostachys pubescens. Results The content of putrescine showed a variety of changes in moso bamboo. In general, the content of putrescine in each tissue was bamboo> bamboo> bamboo whip. The changes of polyamines in different sizes showed different trends. The contents of putrescine decreased gradually with the passage of time, and the content of putrescine increased after the new year of spring bamboo shoots. However, the content of putrescine in juvenile bamboo branches was lower at 4.99 μg / g. The putrescine had a trend of increasing after it entered the year of New Year. The content of putrescine was the lowest in July, 2.50μg / g in July, and it dropped sharply in April of next year with the content of only 1.09μg / g. Conclusion It is significant to explore the effect of polyamines on bamboo bud differentiation. Polyamines can improve bamboo drought resistance, and bamboo can absorb exogenous polyamines. Studying the mechanism of bamboo drought resistance as a growth regulator is also the future research direction.