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在双孢蘑菇同核不育株配对杂交中,发现大约50%的配对组合因为细胞壁不亲和而无法杂交,其中有的组合具有较小的遗传相似性,而且在农学性状上具有潜在的互补优势.为了克服细胞壁亲和性障碍,我们引入原生质体融合技术.由于营养缺陷型或抗药性标记诱变筛选工作烦琐且费时,而且诱变处理改变了亲本的基因结构而可能影响亲本的经济性状.因此,寻找非人工诱变标记来选择、鉴别异核融合株对双孢蘑菇原生质体融合杂交育种具有重大的意义.双孢蘑菇
In a crossbred pair of Agaricus bisporus with a male sterile line, about 50% of the mating combinations were found to be unable to hybridize due to cell wall incompatibility, some of them having less genetic similarity and potentially complementary to agronomic traits Advantages In order to overcome cell-wall affinity disorders, we introduced protoplast fusion technology, which may affect the economic traits of parents due to the cumbersome and time-consuming mutagenesis of auxotrophic or drug-resistant markers and the mutagenesis to alter the genetic structure of the parents Therefore, looking for non-artificial mutagenesis markers to select and identify heterokaryon fusion strains of Agaricus bisporus protoplast fusion breeding is of great significance.Abisporus