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目的：为了探讨ｐ１６基因在喉癌及边缘和声带息肉组织中的表达情况，研究其与喉癌临床间的关系。方法：采用免疫组化ＳＡＢＣ方法对３６例原发喉癌及边缘１．０ｃｍ组织和２０例声带息肉组织中ｐ１６蛋白检测。结果：喉癌组１０例阳性率２７．８％，边缘组２０例阳性率５５．６％，息肉组８例阳性率４０％。喉癌组与边缘组阳性率之间差异显著Ｐ＜０．０５，喉癌组中，阳性率明显高于强阳性率，两者之间差异明显，Ｐ＜０．０５。高分化组阳性率明显高于低分化组阳性率，Ｐ＜０．０５。ｐ１６蛋白的表达在喉癌的临床分期，肿瘤发生部位及是否有淋巴结转移间差异不明显。结论：ｐ１６基因在抑制喉癌细胞增殖分化过程中起着积极的作用
OBJECTIVE: To investigate the expression of p16 gene in laryngeal carcinoma, marginal and vocal cord polyp tissues and to study its relationship with laryngeal cancer. Methods: Immunohistochemical SABC method was used to detect p16 protein in 36 cases of primary laryngeal carcinoma, 1.0cm margin and 20 vocal cord polyps. Results: The positive rate was 27.8% in 10 cases of laryngeal cancer group, 20 cases in marginal group was 55.6%, and in polyp group 8 cases was 40%. There was significant difference between positive rate of laryngeal carcinoma and marginal group (P <0.05). In laryngeal carcinoma group, the positive rate was significantly higher than that of strong positive rate, the difference was significant (P <0.05). The positive rate of well-differentiated group was significantly higher than that of poorly differentiated group (P <0.05). The expression of p16 protein in laryngeal cancer staging, tumor site and whether there is no significant difference between lymph node metastasis. Conclusion: The p16 gene plays a positive role in inhibiting the proliferation and differentiation of laryngeal carcinoma cells