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目的：观察环氧化酶-2（COX－2）在单侧输尿管梗阻小鼠梗阻肾脏内的表达情况，探讨COX-2在炎性肾脏疾病中清理生理作用。方法：利用阿-PCR检测了COX－2在小鼠单侧输尿管梗阻后4h、24h、72h、sd和7d的表达变化并采用PAS染色观察上述各时间点的肾组织病理改变。结果：单侧输尿管梗阻术后4h肾皮质中的COX－2的表达开始增高，72h达到高峰。术后第一天肾间质中可见少量炎细胞浸润，第3d明显增多，第7d达到高峰。结论：本研究提示COX－2的过度表达很可能是单侧输尿管便用后肾内炎细胞浸润和积聚的起站或促进因素之一。
Objective: To observe the expression of cyclooxygenase-2 (COX-2) in obstructive kidneys of unilateral ureteral obstruction mice and to explore the physiological role of COX-2 in inflammatory renal diseases. Methods: The expression of COX-2 in 4h, 24h, 72h, sd and 7d after unilateral ureteral obstruction in mice was detected by A-PCR. The pathological changes of renal tissue were observed by PAS staining. Results: The expression of COX-2 in renal cortex began to increase 4 h after unilateral ureteral obstruction and reached a peak at 72 h. On the first day after operation, a small amount of inflammatory cell infiltration was seen in the renal interstitium, which increased significantly on the 3rd day and peaked on the 7th day. CONCLUSIONS: This study suggests that overexpression of COX-2 is likely to be one of the initiating or contributing factors for unilateral ureter infiltration and accumulation of post-intrarenal nephritis.