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在培养液成分基本不变的情况下,观察几种体外培养条件的间日疟原虫红内裂体增殖.实验组为:烛缸静止培养,重复点烛烛缸静止培养,低氧静止培养,两种试管深层悬浮培养。引种用了两个虫株,起始培养采用冷冻虫血复苏和现症疟疾病人新鲜虫血直接引种。除棉塞试管深层悬浮组不能支持裂体增殖外,其他组均至少不同程度地完成两个裂殖周期;在低氧下,原虫发育更正常。观察发现,间日疟原虫分离株在体外培养中是重要的。
Under the condition of the same culture medium, the proliferation of P.falciparum erythrocytes in vitro was observed in vitro.Experimental groups consisted of static culture of candle jar, static culture of repeated candle candle jar, static culture of hypoxia, Two kinds of test tube deep suspension culture. The introduction of two strains of insects, the initial culture of frozen parasite blood recovery and the disease is now direct introduction of fresh parasites in malaria patients. Except that the deep suspension group in the test tube of tampon could not support the proliferation of split body, other groups all completed the two schizonts at least to some extent. In hypoxia, protozoa developed more normally. It has been observed that P. vivax isolates are important for in vitro culture.