论文部分内容阅读
目的:观察温阳补肾方对小鼠胫骨横行骨折愈合过程的影响。方法:无菌条件下对C57BL/6小鼠实施胫骨骨干横形骨折髓内钉固定术,随机分为模型组和治疗组,治疗组给予温阳补肾方灌胃,模型组给予同体积0.9%氯化钠溶液,分别于给药后第4、7、10、14、21天取样,运用X-ray、Micro-CT、HE染色及形态计量学分析,综合评估温阳补肾方对小鼠胫骨骨折愈合过程的影响并初步探索其作用机制。结果:治疗组骨折线模糊程度在术后14、21d明显高于模型组;术后21d治疗组BV/TV、Tb.N则明显高于模型组(P<0.01),而Tb.Pf、SMI显著低于模型组(P<0.05);术后14、21d治疗组骨性骨痂量、形态、骨小梁方向优于模型组(P<0.05)。结论:温阳补肾方可促进小鼠胫骨横行骨折的愈合,加快对骨痂的再塑形,且其作用时间点为小鼠骨折愈合中后期。
Objective: To observe the effect of Wenyang Bushen Recipe on the healing process of tibial transverse fractures in mice. Methods: C57BL / 6 mice were subjected to intramedullary nailing of transverse tibial shaft fractures under aseptic conditions. The rats were randomly divided into model group and treatment group. The treatment group was given Wenyang Bushen recipe. The model group was treated with 0.9% Sodium chloride solution were taken on the 4th, 7th, 10th, 14th and 21st day after the administration respectively. The effects of Wenyang Bushen Decoction on tibial fractures in mice were evaluated by X-ray, Micro-CT, HE staining and morphometric analysis The healing process and to explore its mechanism of action. Results: The degree of fracture of the fracture line in the treatment group was significantly higher than that in the model group 14 and 21 days postoperatively. BV / TV and Tb.N in the treatment group 21 d after operation were significantly higher than those in the model group (P <0.01) (P <0.05). The amount of bony callus, morphology and trabecular direction in the treatment group at 14 and 21 days after operation were better than those in the model group (P <0.05). Conclusion: Warming Yang and Kidney can promote the healing of transverse tibial fractures in mice and accelerate the remodeling of callus, and its action time point is the middle and late stages of mouse fracture healing.